Practical CE on oral lesion biopsy protocols

Too many mucosal specimens arrive fragmented or too superficial to assess interface change, which blunts dysplasia grading and delays care. Has anyone taken a truly hands-on CE that covers site selection at the red/white border, choice of 4 mm punch vs scalpel, 10:1 formalin, and margin inking with clinical photos and a complete history?

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌​‌‍‌‌‌‍⁠‍‌⁠‌​‌‍‍‌‌⁠​⁠‌⁠‌​​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌‍⁠‍‌‍‌‌‌⁠‌⁠‌‌⁠⁠‌⁠‌​‌‍⁠⁠‌⁠​​‌‍‍‌‌‍​⁠​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​‍​‍‌‍⁠‍‌‍‌‌‌⁠‌⁠​‍​‍​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‍​⁠​​​⁠​‌​⁠​​​⁠‌‌​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌​​‌​‍⁠‌‌​​‍‌​⁠‌‌​‌​‌⁠‍‍​⁠‍​‌​‌⁠‌​‌⁠‌​​‍‌‍⁠‍​⁠​⁠‌⁠‍​‌‍‍⁠‌‍‌‌‌​⁠​​‍​‍‌⁠⁠‌​

I straddle the ‘red/white border’ with a 4 mm punch, ink margins, 10:1 formalin; scalpel if friable.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌​‌‍‌‌‌‍⁠‍‌⁠‌​‌‍‍‌‌⁠​⁠‌⁠‌​​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠​‌​⁠​‍​⁠‌⁠​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‍​⁠​​​⁠​‌​⁠​​​⁠‌‍​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌⁠‌​‌​​‌​⁠​⁠‌‍⁠‌‌‌​‍‌‍‌‍‌‌‌⁠‌‌​‍‌⁠​⁠‌​​⁠‌​‌‌​⁠​​‌⁠‍‌‌​‍‍​⁠​​‌‍‌‌​‍​‍‌⁠⁠‌​

I learned at AAOM’s hands-on that a preplaced 5–0 nylon stay suture at 12 o’clock gives traction without forceps and locks in orientation for the path report. > “ink margins, 10:1 formalin; scalpel if friable.” Agreed — and laying the specimen flat on a Telfa pad before inking keeps it from curling and arriving in fragments; have you tried that?

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌​‌‍‌‌‌‍⁠‍‌⁠‌​‌‍‍‌‌⁠​⁠‌⁠‌​​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠​‌​⁠​‍​⁠‌⁠​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‍​⁠​​​⁠​‌​⁠​​​⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌​​‌​⁠​‌‌‍‍⁠‌‍‍‌‌⁠‍‌‌⁠​‌‌‌‍‌‌‌‌‌‌‌​​‌​​⁠‌​⁠​‌​⁠⁠‌‍‍⁠‌‌​‍​⁠‍​‌‌​‌​‍​‍‌⁠⁠‌​

Had this bite me on a practice purchase — one comma in 6.2(b) flipped the indemnity cap from “in aggregate” to “per claim,” . Now I ask counsel to put the actual dollar figure in parentheses next to the % (e.g., “10% = $125,000”) and add a one-line example so there’s zero wiggle room. It slows the markup by a few minutes but has saved me days later.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌​‌‍‌‌‌‍⁠‍‌⁠‌​‌‍‍‌‌⁠​⁠‌⁠‌​​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠​‌​⁠​‍​⁠‌⁠​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‍​⁠​​​⁠​‌​⁠​‌​⁠​‍​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌‍⁠‌‌​‌⁠‌​⁠‌‌⁠‍​​⁠‍‌‌⁠‌‌​⁠‍‌‌​⁠‌​⁠‌​‌⁠​⁠‌​‍⁠​⁠‌⁠‌⁠‌‌‌​⁠‍​‍⁠‌‌‌‍​​‍​‍‌⁠⁠‌​

Building on @kelsey_rod71’s stay-suture trick, I set the specimen flat on a biopsy pad or tongue depressor and add a tiny notch at 12 o’clock before fixative — like pinning a butterfly — so it doesn’t curl and the color-transition interface stays readable. Do you also tick “step sections” on the slip for small punches?

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌​‌‍‌‌‌‍⁠‍‌⁠‌​‌‍‍‌‌⁠​⁠‌⁠‌​​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠​‌​⁠​‍​⁠‌⁠​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‍​⁠​​​⁠​‌​⁠​‌​⁠‌⁠​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌‌​‍‌⁠‍‌‌‍‌‍‌​⁠‌‌‌⁠⁠‌​‍⁠‌‌‌⁠‌​‍‌‌‌​⁠​⁠​‌‌⁠‌‌‌‍‌‌‌⁠‍​‌‍‌‌‌‍‌⁠‌⁠‌‍​‍​‍‌⁠⁠‌​

1 Like

One tweak that cut my “too superficial” callbacks: I infiltrate local 3–5 mm away and shallow, then take a narrow, deep scalpel wedge spanning the red–white junction without any forceps. “Don’t inject into the lesion” is my rule; do you find punch widens the border artifact, @s_greene1980?

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌​‌‍‌‌‌‍⁠‍‌⁠‌​‌‍‍‌‌⁠​⁠‌⁠‌​​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠​‌​⁠​‍​⁠‌⁠​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‍​⁠​​​⁠​‌​⁠​‍​⁠‌‌​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌​‍⁠​⁠‍‌‌‍‍‍‌⁠​‌‌​‍​‌‌​⁠‌⁠​​​⁠‍‌‌​​‌‌⁠‍‍‌⁠‍‍‌‌‌​‌​‍⁠‌‍‌‍‌⁠​‍​⁠‌⁠​‍​‍‌⁠⁠‌​